BLV/HTLV Genomics

We are developing and applying state-of-the-art genomic technologies to study the BLV-in-sheep model system in order to gain a detailed understanding of BLV and HTLV-1-induced leukemogenesis.  We have discovered a cluster of miRNAs in the BLV genome that has a major impact on disease progression by yet unknown mechanisms, and have shown that BLV/HTLV-1 proviral integration sites are not random and participate in early clonal expansion/persistence by perturbing neighbouring cancer drivers.    

Key publications

Pooled CRISPR Inverse PCR sequencing (PCIP-seq): simultaneous sequencing of retroviral insertion points and the associated provirus in thousands of cells with long reads. Artesi M$, Hahaut V$, Ashrafi F, Marçais A, Hermine O, Griebel P,  Arsic N, van der Meer F, Burny A, Bron D, Charlier C, Georges M, Van den Broeke A#, Durkin K#

Monitoring Molecular Response in Adult T-cell Leukemia/Lymphoma by High-Throughput Sequencing Analysis of HTLV-1 Clonality. Artesi M, Marçais A, Durkin K, Rosewick N, Hahaut V, Suarez P, Trinquand A, Lhermitte L, Avettand Fenoel V, Georges M, Hermine O & Van den Broeke ALeukemia 31: 2532-2535 (2017)

Cis-perturbation of cancer drivers by the HTLV-1/BLV proviruses is an early determinant of leukemogenesis. Rosewick N, Durkin K, Marçais A, Artesi M, Hahaut V, Griebel P, Arsic N, Avettan-Fenoel V, Burny A, Charlier C, Hermine O, Georges M, Van den Broeke ANat Commun 8: 15264 (2017)

Deep sequencing reveals abundant class of retroviral microRNAs in BLV-induced leukemia. Rosewick N, Durkin K, Momont M, Takeda H, Caiment F, Cleuter Y, Vernin C, Mortreux F, Wattel E, Burny A, Georges M, Van den Broeke A.  Proc Natl Acad Sci USA 110:2306-2311 (2013).

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